Validation of a quantitative analysis method for carvacrol by HPLC-DAD in Origanum vulgare L. veterinary cream 2% (m/m)
DOI:
https://doi.org/10.25758/set.953Keywords:
Origanum vulgare L., HPLC-DAD, Carvacrol, Cream, Quality controlAbstract
Introduction – The use of creams containing essential oils from aromatic plants is a growing area in veterinary medicine. Essential oils contain biological properties, including antimicrobial, antioxidant, and anti-inflammatory activities, which can support the treatment of skin problems in cats and dogs. Quality control of creams containing Origanum vulgare L. essential oil includes, among other analyses, quantification of carvacrol, a major phenolic monoterpene. Objectives – This study aims to extract, separate, and quantify the carvacrol present in a cream formulation, thereby validating this quality control method. Methods – Three consecutive solid-liquid extractions were performed on 100mg of cream, yielding a distribution of extracted carvacrol quantities of 74.7%, 19.4%, and 5.9%, respectively. This corresponded to a total recovery of 95% of the essential oil content in the cream. The extracts were analyzed by HPLC-DAD on an RP-C18 column with a mobile phase of methanol:water:acetonitrile. Results – The method was validated, resulting in intra-assay and inter-assay precision values below 2% RSD. The LOD was found to be 0.65µg/mL, and the LOQ was 1.95µg/mL. Carvacrol constituted approximately 36% of the mass of the main chemotype's essential oil. Conclusions – The validated method is suitable for quality control of creams containing carvacrol and may be adapted for analysis of other monoterpenes.
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