Detection and quantification of genetically modified soy in foodstuffs, commercialized in Portugal, for human consumption

Authors

  • Rita Faustino Área Científica de Dietética, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa. Lisboa, Portugal.
  • Ana Sousa Área Científica de Dietética, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa. Lisboa, Portugal.
  • Marta Loureiro Área Científica de Dietética, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa. Lisboa, Portugal.
  • Lino Mendes Área Científica de Dietética, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa. Lisboa, Portugal.
  • Miguel Brito Área Científica de Biologia, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa. Lisboa, Portugal.

DOI:

https://doi.org/10.25758/set.214

Keywords:

Detection, Quantification, Genetically modified soy, GMO, Real-time PCR, RT-PCR

Abstract

Biotechnology allows plant and animal genetic modification, leading to genetically modified organisms (GMO). The GMO-related benefits and risks are still surrounded by controversy, the reason why the consumer has the right to decide about its consumption. The aim of this work is to detect and quantify genetically modified soy in foodstuffs. In that sense, 11 samples from two different lots of the same foodstuff’s brand, which contain soy, for human consumption were analysed. The real-time PCR method was used to detect and quantify transgenic DNA. From all the samples analysed, 7 were negative (63.6%) and 4 positives (36.4%) for the DNA detection from transgenic soy, corresponding to 3 foodstuffs: soy milk, flour, and granulated. The DNA quantities from transgenic soy vary from 0.07% (granulated soy) to 0.22% (soy flour), suggesting environmental contamination because they are below 0.4%. 

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Published

2009-05-15

Issue

Section

Artigos

How to Cite

Detection and quantification of genetically modified soy in foodstuffs, commercialized in Portugal, for human consumption. (2009). Saúde & Tecnologia, 03, 19-24. https://doi.org/10.25758/set.214