Manchas de sangue seco em papel filtro: uma opção válida para extraçãode DNA?
DOI:
https://doi.org/10.25758/set.729Keywords:
Extração de DNA, Manchas de sangue seco, Condições de armazenamento, Amostras de sangueAbstract
As manchas de sangue seco parecem ser uma opção válida para a recolha de amostras para extração de DNA, especialmente em comunidades mais pobres e com menos acesso a cuidados médicos. As manchas de sangue seco podem ser facilmente transportadas e garantem um método não invasivo de colheita rápida que pode ser acoplado a métodos de extração de DNA.
No entanto, as condições de armazenamento e transporte (tempo e temperatura), bem como o tipo e volumes de amostra podem condicionar a eficiência da extração de DNA e tornar-se um desafio. Neste contexto avaliaram-se os efeitos na eficiência da extração de DNA de seis condições de armazenamento (24 horas a 4 °C, 24 horas à temperatura ambiente, 24 horas a 50 °C, sete dias a 4 °C, sete dias à temperatura ambiente temperatura e sete dias a 50 °C) para amostras de sangue seco com sangue venoso e amostras de sangue capilar. Para avaliar a proficiência na extração de DNA, em cada uma dessas condições, realizou-se a extração de DNA com o QIAamp® Blood Mini Kit e avaliaram-se as concentrações finais e taxas de pureza do DNA, por meio de espectrofotometria UV com auxílio do Nanodrop® OneC. A análise estatística foi realizada por meio do teste T no Microsoft® Excel. Os resultados obtidos demonstraram que é possível extrair uma quantidade maior de DNA de amostras de sangue capilar em amostras de sangue seco, embora com menor pureza do que amostras de sangue venoso. Além disso, os resultados mostram que a temperatura
avaliada e o tempo de armazenamento (sete dias) não afetam os resultados da extração de DNA.
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