Dry blood spots in filter paper: a valuable option for DNA extraction?
DOI:
https://doi.org/10.25758/set.729Palavras-chave:
Dry extraction, Dry blood spots, Storage conditions, Blood samplesResumo
Dry blood spots, appear to be a valuable option for sample collection for DNA extraction, especially in poorer communities with less access to medical care. Dry blood spots can be easily transported and ensure a non-invasive method of rapid blood collection which can be coupled with DNA extraction methods. However, the storage and transportation conditions (time and temperature) as well as sample type and volumes may affect DNA extraction efficiency and become a challenge. In this context, we aim to assess the effects on DNA extraction efficiency of six storage conditions (24 hours at 4 °C, 24 hours at room temperature, 24 hours at 50 °C, seven days at 4 °C, seven days at room temperature, and seven days at 50 °C) for dry blood spots with venous blood and capillary blood samples. In order to evaluate DNA extraction proficiency, under each of these conditions, we performed DNA extraction with the QIAamp® Blood Mini Kit and assessed DNA final concentrations and purity ratios, through UV spectrophotometry, with the aid of the Nanodrop® OneC. Statistical analysis was performed using a T-test in Microsoft® Excel. Overall, our data demonstrated that it is possible to extract a greater DNA quantity from capillary blood samples in dry blood spots, although with less purity than venous blood samples. Also, our results show that the assessed temperature and storage time (seven days) do not affect DNA extraction results.
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